Applications for Cell Culture Analysis
Simple Evaluation of Cultured Cells: Evaluation of Differentiation State of iPS Cells Using Benchtop MALDI-TOF MS
The growth condition and differentiation state (differentiated or undifferentiated) of cultured cells are important evaluation items in regenerative medicine and basic research. If these cell evaluations can be conducted in a simple manner by mass spectrometry, evaluation time and complicated work can be significantly reduced. Therefore, in order to examine the possibility of determining the state of cells by mass spectrometry, we conducted an automatic analysis of iPS cells and their culture media by AuraSolution™ using the Shimadzu MALDI-8020 benchtop linear MALDI-TOF mass spectrometer, and attempted a preliminary analysis by the statistical analysis software eMSTAT Solution™.
Evaluation of Differentiated/Undifferentiated State of iPS Cells Using DPiMS-2020 and eMSTAT Solution
This article introduces an example of quick and simple measurement of the components contained in iPS cell culture medium with a DPiMS-2020 mass spectrometer using the probe electro spray ionization method, which is a new ionization method. This article also introduces an example in which grouping of the culture media of differentiated/undifferentiated cells was possible by using eMSTAT Solution statistical analysis software.