Cell Culture & Cell Culture Media Analysis / C2MAP


One of such specialized solution is the C2MAP system. You can find out more details about the system here.


Cell Culture
Special cell culture media analysis platform


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Applications for C2MAP


A Novel Cell Culture Media Analysis Platform for Culture Process Development

Optimization and control of cell culture processes are essential to increase production efficiency of biopharmaceuticals. In the field of cell therapy including regenerative medicines, enhanced control of the culture process is also becoming important to reduce cell variability and improve consistency of mass production of the cells. Comprehensive monitoring of culture supernatant components gives researchers useful information for these purposes. However, current technologies for process monitoring are limited to measurement of pH, dissolving gases, and some small compounds such as glucose, glutamine, lactate, and ammonia in culture supernatant. We have developed a “Cell Culture Media Analysis Platform, C2MAP system” that combines automated pretreatment module for culture supernatant samples with LC/MS/MS.



Evaluation of Undifferentiated State of Human iPS Cells using C2MAP Cell Culture Media Analysis Platform

Development of technologies for the preparation and supply of iPS cells with high quality and a large amount is an essential requirement for commercialization of regenerative medicine. Conventionally, manual techniques such as Gene Expression analysis had been used to evaluate the status of cell differentiation, but because these are invasive techniques that cause cell disruption, they were generally applied as evaluation methods after culturing was completed.



Comprehensive Cell Culture Profiling Using the LCMS-9030 Quadrupole TOF Mass Spectrometer

In the production of useful substances by fermentation and the manufacturing of antibody drugs, the monitoring of culture media pH, dissolved gases, carbon sources (glucose), and nitrogen sources (glutamine) is implemented in order to optimize and manage the cultivation process. In addition to glucose and glutamine, the culture media components in cell cultures comprise vitamins and nucleic acid related compounds as well as metabolites and other compounds secreted from cells. Accordingly, the comprehensive analysis of compounds contained culture media is expected to provide useful information for discussing bioprocesses.

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