Columns for SFC

Features

 

When conducting analysis with the Nexera UC supercritical fluid chromatography system, because diffusion of the sample band in the mobile phase is high compared with liquid chromatography, separation behavior changes significantly depending on the types of columns used.
Shim-pack UC-X series was designed with a variety of stationary phases, making it suitable for analysis of various compounds.

 

Achieve high speed and high performance
 

Shim-pack UC-X series offers various stationary phases suitable for the mobile phase of high diffusion and low-viscosity liquid carbon dioxide.
Analysis time can be reduced at a high flow rate without impairing the separation performance. In addition, analysis speed, which is limited in HPLC, can be increased.

 

Wide range of stationary phases meets diverse needs
 

Shim-pack UC-X series was designed with eight types of stationary phases and sizes to meet diverse research and development needs.

 

High durability and stable reproducibility
 

Shim-pack UC-X series achieves high inertness for improved analysis precision and increased column durability. Its uniform silica surface and stable chemical modification also ensure high analysis reproducibility.

 

Specifications
 

  Shim-pack
UC-X RP
Shim-pack
UC-X GIS II
Shim-pack
UC-X Phenyl
Shim-pack
UC-X CN
Bonded Phase Octadecyl Groups +
Polar Functional Group
Octadecyl Groups Phenyl Groups Cyanopropryl Groups
Analysis Mode Reversed Phase Reversed Phase Reversed Phase Normal Phase, Reversed Phase
Features Unique selectivity High inertness Strong π-π interactions Suitable in either reversed phase or normal phase mode
Particle Size (μm) 3, 5 3, 5 3, 5 3, 5
Max. Operating Pressure (MPa) 20 20 20 20
Pore Size (nm) 10 10 10 10
Surface Area (m2/g) 450 450 450 450
Carbon Loading (%) 9 11 9.5 14
Pore Volume (mL/g) 1.05 1.05 1.05 1.05
End Cap - - -
pH Range 2 - 7.5 2 - 7.5 2 - 7.5 2 - 7.5
USP Code L1 L1 L11 L10

 

  Shim-pack
UC-X Diol
Shim-pack
UC-X Sil
Shim-pack
UC-X Amide
Shim-pack
UC-X NH2
Bonded Phase Diol Group - Carbamoyl Groups Aminopropyl Groups
Analysis Mode Normal Phase Normal Phase HILIC HILIC, Normal Phase
Features High retentive in normal phase mode Ultra-pure silica gel Strong retention of highly polar compounds Sugar analysis
Particle Size (μm) 3, 5 3, 5 3, 5 3, 5
Max. Operating Pressure (MPa) 20 20 20 20
Pore Size (nm) 10 10 10 10
Surface Area (m2/g) 450 450 450 450
Carbon Loading (%) 20 - 18 8
Pore Volume (mL/g) 1.05 1.05 1.05 1.05
End Cap - - - -
pH Range 2 - 7.5 2 - 7.5 2 - 7.5 2 - 7.5
USP Code L20 L3 - L8

 

Analysis Examples

 

Tocopherol isomers
 

Isomers and structurally similar compounds that are difficult to separate can be analyzed by Nexera UC and Shim-pack UC-X series at high speed. In this example, four kinds of tocopherol isomers were analyzed by Nexera UC and Shim-pack UC-X Sil. As indicated on the right, analysis time was reduced to one-third the time achieved with conventional HPLC method while resolution was improved.

 


Comparison of retention and resolution of tocopherol isomers between LC condition and SFC ones

 

 

Phospholipid classes
 


Analysis of phospholipid classes

  The example on the left shows the result of the analysis of phospholipid classes using Shim-pack UC-X Diol with Nexera UC. The ODS-based Shim-pack UC-X GIS II can also be used to analyze molecular species of phospholipids with the same modifiers. Nexera UC and Shim-pack UC-X series are ideal for analyzing complex samples.

 

Multiple pesticides with wide polarity range
 

Simultaneous analysis of multiple pesticides with a wide polarity range from hydrophobic to hydrophilic ones has been achieved using the Nexera UC using Shim-pack UC-X RP.
Shim-pack UCX-RP contains a polar functional group embedded between the silica surface and the C18 group. This allows the column to analyze a wide range of components from hydrophobic to hydrophilic ones.
Simultaneous analysis of multiple components can now be performed more efficiently using the Nexera UC and Shim-pack UC-X series.

 

 
No. components log P
1 Carbofuran 7.4 
2 Ethofenprox 6.9 
3 Fenpropathrin 6.0 
4 Pyriproxyfen 5.0 
5 Pyraclostrobin 4.0 
6 Linuron 3.0 
7 Aminocarb 1.9 
8 Ethoxysulfuron 1.0 
9 Halosulfuron methyl 0.0 
10 Bentazone -0.5 
11 Chlorsulfuron -1.0 
12 Rimsulfuron -1.5 
13 Nicosulfuron -1.8 
14 Vamidothion -4.2 

 

Lineup

 

Product Name I.D. (mm) Length (mm) Particle Size
3 μm 5 μm
Shim-pack UC-X RP 2.1 150 227-30500-01 227-30502-01
2.1 250 227-30500-02 227-30502-02
4.6 150 227-30501-01 227-30503-01
4.6 250 227-30501-02 227-30503-02
Shim-pack UC-X GIS II 2.1 150 227-30504-01 227-30506-01
2.1 250 227-30504-02 227-30506-02
4.6 150 227-30505-01 227-30507-01
4.6 250 227-30505-02 227-30507-02
Shim-pack UC-X Diol 2.1 150 227-30508-01 227-30510-01
2.1 250 227-30508-02 227-30510-02
4.6 150 227-30509-01 227-30511-01
4.6 250 227-30509-02 227-30511-02
Shim-pack UC-X Sil 2.1 150 227-30512-01 227-30514-01
2.1 250 227-30512-02 227-30514-02
4.6 150 227-30513-01 227-30515-01
4.6 250 227-30513-02 227-30515-02
Shim-pack UC-X Amide 2.1 150 227-30516-01 227-30518-01
2.1 250 227-30516-02 227-30518-02
4.6 150 227-30517-01 227-30519-01
4.6 250 227-30517-02 227-30519-02
Shim-pack UC-X NH2 2.1 150 227-30520-01 227-30522-01
2.1 250 227-30520-02 227-30522-02
4.6 150 227-30521-01 227-30523-01
4.6 250 227-30521-02 227-30523-02
Shim-pack UC-X Phenyl 2.1 150 227-30524-01 227-30526-01
2.1 250 227-30524-02 227-30526-02
4.6 150 227-30525-01 227-30527-01
4.6 250 227-30525-02 227-30527-02
Shim-pack UC-X CN 2.1 150 227-30528-01 227-30530-01
2.1 250 227-30528-02 227-30530-02
4.6 150 227-30529-01 227-30531-01
4.6 250 227-30529-02 227-30531-02

 

 

For Research Use Only. Not for use in diagnostic procedures.

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