DNA-12000 reagent kit

DNA-12000 reagent kit

Suited for research of cloning a gene and gene expression

 

MultiNA, which realizes easier, faster, and more sensitive electrophoresis than agarose gel, enables a wide range of DNA size analysis to 12 kbp.
Expanded applications



Check the size of plasmid DNA which is digested with a restriction enzyme.



Check the size and presence or absence of a cloning gene with Plasmid DNA (insert check)



Check the size and presence or absence of an amplified long PCR product from a gene.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Application

MultiNA: Application for Gene Cloning

 

Overview

 Description  DNA-12000 Kit
 P/N  292-36600-91
 Contents  Separation buffer for 1000 analyses,

 marker solution for 1000 analyses,

 buffer bottle, vials, instruction manual
 Storage

 temperature
 Separation buffer: 2 to 8 °C

 Marker solution: -20 °C

 

The kits do not contain fluorescent dyes or ladders. This kit has a shelf life. Please use it immediately after opening.

 

Specifications

 Size range  100 to 12,000 bp
 Sizing resolution  10 % (100 to 1,000 bp), 20 % (1,000 to 12,000 bp)
 Sizing accuracy  ±15 %
 Quantitative range  0.5 to 50 ng/μL
 Quantitative accuracy  ±40% (The quantitative accuracy was verified from 200 to 12000 bp.)
 Maximum salt concentration  125mM KCl

* The specifications above were verified using standard samples and analysis conditions prescribed by Shimadzu.

  The specifications may not be achieved for some samples and/or analysis conditions.

Operation environment

MCE-202 MultiNA software Ver. 1.10 or later is required to use the DNA-12000 kit.
  • Upgrade software
  • Upgrade details differ between software Ver. 1.05 or later and Ver. 1.06.

    For more information, please contact your local Shimadzu distributor.

Other reagent kits for MultiNA

 Description  Separation range
 DNA-500 Kit (1,000 analyses)  25 to 500 bp
 DNA-1,000 Kit (1,000 analyses)  100 to 1,000 bp
 DNA-2,500 Kit (1,000 analyses)  100 to 2,500 bp
 RNA Kit (1,000 analyses)  Max. 28rRNA (5.0 knt)

 

 

Features of the MCE-202 MultiNA microchip electrophoresis system

 

  1. No gel creation required and automated analysis of up to 108 loaded samples.
  2. High repeatability of predicted size values.
  3. High-sensitivity detection without the use of ethidium bromide.
  4. Viewer allows parallel display of analysis data from different times and dates.
 

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