DNA-12000 reagent kit
DNA-12000 reagent kit
Suited for research of cloning a gene and gene expression
MultiNA, which realizes easier, faster, and more sensitive electrophoresis than agarose gel, enables a wide range of DNA size analysis to 12 kbp.
Expanded applications
Check the size of plasmid DNA which is digested with a restriction enzyme.
Check the size and presence or absence of a cloning gene with Plasmid DNA (insert check)
Check the size and presence or absence of an amplified long PCR product from a gene.
Application
MultiNA: Application for Gene Cloning
Overview
| Description | DNA-12000 Kit |
| P/N | 292-36600-91 |
|---|---|
| Contents | Separation buffer for 1000 analyses, marker solution for 1000 analyses, buffer bottle, vials, instruction manual |
| Storage temperature | Separation buffer: 2 to 8 °C Marker solution: -20 °C |
The kits do not contain fluorescent dyes or ladders. This kit has a shelf life. Please use it immediately after opening.
Specifications
| Size range | 100 to 12,000 bp |
|---|---|
| Sizing resolution | 10 % (100 to 1,000 bp), 20 % (1,000 to 12,000 bp) |
| Sizing accuracy | ±15 % |
| Quantitative range | 0.5 to 50 ng/μL |
| Quantitative accuracy | ±40% (The quantitative accuracy was verified from 200 to 12000 bp.) |
| Maximum salt concentration | 125mM KCl |
* The specifications above were verified using standard samples and analysis conditions prescribed by Shimadzu.
The specifications may not be achieved for some samples and/or analysis conditions.
Operation environment
MCE-202 MultiNA software Ver. 1.10 or later is required to use the DNA-12000 kit.
- Upgrade software
- Upgrade details differ between software Ver. 1.05 or later and Ver. 1.06.
For more information, please contact your local Shimadzu distributor.
Other reagent kits for MultiNA
| Description | Separation range |
|---|---|
| DNA-500 Kit (1,000 analyses) | 25 to 500 bp |
| DNA-1,000 Kit (1,000 analyses) | 100 to 1,000 bp |
| DNA-2,500 Kit (1,000 analyses) | 100 to 2,500 bp |
| RNA Kit (1,000 analyses) | Max. 28rRNA (5.0 knt) |
Features of the MCE-202 MultiNA microchip electrophoresis system
- No gel creation required and automated analysis of up to 108 loaded samples.
- High repeatability of predicted size values.
- High-sensitivity detection without the use of ethidium bromide.
- Viewer allows parallel display of analysis data from different times and dates.
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