To start analysis without an investigation of measurement conditions, select a method suited to the analysis aims and the relevant compounds. Protocols are also included for extraction from biological tissue, plasma, and feces, as well as derivatization and other pretreatments.
Example Analysis: Intestinal Flora Research
Recently the analysis of metabolites in the field of intestinal flora has expanded in scope. Comprehensive analysis includes not only general primary metabolites, but D-amino acids and other chiral molecules as well as lipids and bile acids.
Measurements of mouse feces using this product can detect 554 compounds, as shown in the table.
Number of compounds detected by each method
LCMS Primary Metabolites
PFPP Method: 71 compounds
Ion Pair Method: 35 compounds
GCMS Primary Metabolites
DL Amino Acids
D-Amino Acids: 17 compounds
L-Amino Acids: 21 compounds
Short-Chain Fatty Acids
Multi-omics Analysis Package
With the included Multi-omics Analysis Package, it is possible to display quantitative fluctuations in metabolites on a metabolic map, and to visualize relationships between metabolites.