LC/MS/MS Method Package for Primary Metabolites
Ready-to-Use Analytical Conditions
Shimadzu Method Packages deliver conditions for efficient and simultaneous multi-component analysis. They enable the user to quickly and easily implement complex methods without costly and laborious method development by providing sample preparation protocols, LC separation conditions, and MS acquisition parameters.
Ion pair or PFPP column method
A PFPP: Pentafluorophenylpropyl column method was newly adapted to expand your choice in separation conditions as well as target compounds.
Supporting LCMS-8030/8040/8050
This method package supports the latest Shimadzu UFMS triple quadrupole mass spectrometer series, LCMS-8030, 8040 and 8050. LCMS-8050 delivers best-in-class sensitivity and the fastest scanning and polarity switching rates.
Complete from Sample Preparation to Analysis
Protocols are included for the preparation of extracts from biological tissue. Saving the user time and money, even laboratories unfamiliar with extraction can follow prescribed steps for LC/MS/MS sample preparation.
Normalization of Multiple Sample Results
Due to mass differences between biological tissue samples, normalization of results must be performed when multiple samples are analyzed. This method package includes optimized analytical conditions for two internal standards to permit normalization across multiple samples.
Area ratio comparison between two tissues focused on methylation cycle
| List of compounds for ion pair method | |
|---|---|
| Glycolytic system | Nucleotides |
| 2,3-Bisphosphoglyceric acid 3-Phosphoglyceric acid (2-Phosphoglyceric acid) Dihydroxyacetone phosphate Fructose 1,6-bisphosphate Glucose 1-phosphate Glucose 6-phosphate Glycerol 3-phosphate Phosphoenolpyruvic acid Pyruvic acid |
Adenosine 3',5'-cyclic monophosphate Adenosine diphosphate Adenosine monophosphate Adenosine triphosphate Cytidine diphosphate Cytidine monophosphate Cytidine triphosphate Guanosine 3',5'-cyclic monophosphate Guanosine diphosphate Guanosine monophosphate Guanosine triphosphate Thymidine diphosphate Thymidine monophosphate Thymidine triphosphate Uridine diphosphate Uridine monophosphate Uridine triphosphate |
| Co-enzyme | Pentose-phosphate pathway |
| NAD NADH NADP NADPH |
|
| 6-Phosphogluconic acid Erythrose 4-phosphate Ribose 5-phosphate Ribulose 5-phosphate Sedoheptulose 7-phosphate |
|
| TCA cycle | Internal STDs |
| Acetyl coenzyme A Succinyl coenzyme A |
2-Morpholinoethanesulfonic acid Methionine sulfone |
| Amino acids | |
| Alanine Arginine Asparagine Aspartic acid Cysteine Glutamic acid Glutamine Glycine Histidine Lysine Methionine Phenylalanine Serine Threonine Tryptophan Tyrosine |
|
| List of compounds for PFPP column method | |
|---|---|
| Glycolytic system | Organic acids |
| Lactic acid Pyruvic acid |
4-Aminobutyric acid Adenylsuccinic acid Argininosuccinic acid Cholic acid Creatine Nicotinic acid Ophthalmic acid Orotic acid Pantothenic acid Taurocholic acid Uric acid |
| TCA cycle | |
| 2-Ketoglutaric acid Aconitic acid Citric acid Fumaric acid Isocitric acid Malic acid Succinic acid |
|
| Amino acids | Nucleosides and Nucleotides |
| 4-Hydroxyproline Alanine Arginine Asparagine Aspartic acid Asymmetric dimethylarginine Citrulline Cystine Dimethylglycine Glutamic acid Glutamine Glycine Histidine Homocystine Isoleucine Leucine Lysine Methionine sulfoxide Ornitine Phenylalanine Proline Serine Symmetric dimethylarginine Threonine Tryptophan Tyrosine Valine |
Adenine Cytosine Guanine Thymine Uracil Xanthine Adenosine Cytidine Guanosine Inosine Thymidine Uridine Adenosine 3',5'-cyclic monophosphate Adenosine monophosphate Cytidine 3',5'-cyclic monophosphate Cytidine monophosphate Guanosine 3',5'-cyclic monophosphate Guanosine monophosphate Thymidine monophosphate |
| Co-enzymes | |
| FAD FMN NAD |
|
| Methylation and Transsulfuration cycle | Others |
| Cystathionine Cysteine Homocysteine Methionine |
2-Aminobutyric acid Acetylcarnitine Acetylcholine Allantoin Carnitine Carnosine Choline Citicoline Creatinine Cysteamine Dopa Dopamine Epinephrine Histamine Hypoxanthine Kynurenine Niacinamide Norepinephrine Serotonin |
| 5-Glutamylcysteine Glutathione Oxidized glutathione S-Adenosylhomocysteine S-Adenosylmethionine |
|
| Internal STDs | |
| 2-Morpholinoethanesulfonic acid Methionine sulfone |
|
Remarks and Precautions:
- For Research Use Only. Not for Use in Diagnostic Procedures.
- Shimadzu makes no warranty regarding the accuracy of information included in the database or the usefulness of information obtained from using the database.
- It is the user’s responsibility to adopt appropriate quality control tests using standard samples to confirm qualitative and quantitative information obtained with this method package.