"Collective Analysis" mode
Automatically obtain MS images with distinctive characteristics thanks to the "Collective Analysis" mode with pre-set parameters.
This is a highly convenient feature when you need to process a lot of data in the same way. The user can create data matrix tables from differential analysis and/or image analysis, carry out statistical data analysis, and obtain MS images all in one step.
Examples using "Collective Analysis"
Using the "Collective Analysis" mode, MS images where the NASH tissue shows peculiarities with respect to normal tissue are automatically selected.
Introducing an analysis of mouse liver for NASH (Non-Alcoholic Steatohepatitis) using 3 examples of analysis modes.
- 1. Finding molecules unique to NASH tissue (Differential Analysis)
- 2. Finding molecules with the same distribution as the stained image (Image Analysis)
- 3. Creating an MS image showing the concentration distribution of the target molecule (Quantitative Analysis)
What is NASH?
NASH (Non-Alcoholic Steatohepatitis) refers to one type of fatty liver disease that is not related to alcohol consumption.
1. Finding molecules unique to NASH tissue Differential Analysis
Using statistical methods and comparing the average spectra of the ROIs, find molecules that are causing the differences between the ROIs.
Further analysis can be carried out using the test functions, PLS (partial least squares) function, etc.
2. Finding molecules with the similar distribution as the stained image Image Analysis
Using statistical methods and comparing each m/z distribution, find molecules with distinctive distributions.
Further data analysis can be carried out using "Image Classification", "Segmentation", etc.
3. Creating an MS image showing the concentration distribution of the target molecule Quantitative Analysis
Using calibration curves, convert the intensity of the target molecule in the ROI into a concentration value.
Convert data files to imdx format readable by the IMAGEREVEAL MS using the included "IMDX Converter" tool.
The iMScope "kbd" format and general "Analyze 7.5" and "imzML" can be converted with this tool.
Target analysis / Non-target analysis
- Target analysis Analysis of only target m/z values based on a list of e.g. lipids or metabolites. It is also possible to create a custom list.
- Non-target analysis Analysis over all m/z within the specified MS range. Useful for checking which distinctive m/z values are contained within the range.
This is a table containing information such as compound names, m/z values, and adduct ions. Using this table, you can associate compounds with m/z values to carry out MS image analysis.
Simultaneous processing of multiple MS Image data files
It is possible to process data from multiple data files all together, and by inputting all the data at once, it is simple to compare the images. Data analysis can be carried out on up to a few hundred GB without splitting up the data.