Isocratic System
Features
Analysis of PTH-Amino Acids Using Isocratic Mode
Performing PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.
Simple Operations
Specialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.
Analytical Stability and Higher Detection
Baseline Stability
PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.
Retention Time Reproducibility
Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.
Higher Detection
Cycle 10 PTH-Val
PPSQ-51A/53A
PPSQ-31B/33B
Cycle 21 PTH-Ile
PPSQ-51A/53A
PPSQ-31B/33B
Specifications
| Main Unit | PPSQ-51A/53A |
|---|---|
| Reaction method | Edman degradation |
| Reaction time | PPSQ-51A: 46.5 min/cycle |
| PPSQ-53A: 48 min/cycle | |
| Number of reactors | PPSQ-51A: 1 |
| PPSQ-53A: 3 | |
| Sample immobilization method | Glass fiber disk (8 mm dia.) or PVDF membrane |
| Reactor temperature control range | 10 °C above room temperature to 60 °C |
| Converter temperature control range | 10 °C above room temperature to 70 °C |
| Number of reagents/solvents | 7 |
| Reagent/solvent delivery method | Nitrogen gas pressure |
| Dimensions | W510 x D500 x H540 mm |
| Weight | PPSQ-51A: 43 kg PPSQ-53A: 45 kg |
| Solvent Delivery Module | LC-20AT |
|---|---|
| Flow rate setting range | 0.001 to 10.000 mL/min |
| Dimensions | W260 x D420 x H140 mm |
| Weight | 11 kg |
| Detector | SPD-M30A |
|---|---|
| Wavelength setting range | 190 to 700 nm |
| Dimensions | W260 x D500 x H140 mm |
| Weight | 12 kg |
| Oven | CTO-20A |
|---|---|
| Temperature control range | 10 °C above room temperature to 60 °C |
| Dimensions | W260 x D420 x H415 mm |
| Weight | 20 kg |
| Control PC | |
|---|---|
| OS | Windows 7 Professional SP1 32/64 bit |
Note: In the interests of product improvement, these specifications may change without notice.
Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.
Standard Configurations
PPSQ-51A System
| Configuration | PPSQ-51A (main unit) |
|---|---|
| LC-20AT | |
| SPD-M30A | |
| CTO-20A |
PPSQ-53A System
| Configuration | PPSQ-53A (main unit) |
|---|---|
| LC-20AT | |
| SPD-M30A | |
| CTO-20A |
Other Items Provided by User
| Installation space | Desktop: W1,600 x D600 x H800 mm min. |
|---|---|
| Weight: Approx. 100 kg | |
| Power supply | 120-230 VAC, 50/60 Hz, 1,500 VA max. |
| Nitrogen gas | Purity: 99.9999% min. |
| A cylinder pressure regulator and gas tubing (10 m) are provided as standard accessories. | |
| Exhaust equipment | In order to provide an exhaust for gases produced by waste liquids, an exhaust tube must either be connected to exhaust equipment or vented outside. An exhaust tube (20 m) is provided as a standard accessory. |
Note: The required installation space and power supply may change according to the PC and display used.
Installation Example
PPSQ-51A Single Reactor
Offers highly cost-effective performance.
PPSQ-53A Triple Reactor (Supports consecutive analysis of 3 samples.)
Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.
For Research Use Only. Not for use in diagnostic procedures.
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