Preparative system general HPLC: Nexera Prep

Significantly Reduces the Processes Involved from Preparative Work to Purification (Free basing Treatment) and Powderization

With conventional preparative methods, the fractionated sample contains mobile phase solvent, which leads to unavoidable dilution during fractionation, so the fractionated sample must be enriched and desalted. Such procedures complicate the workflow, and amplify sample loss, labor, and costs.
With the Shimadzu UFPLC Ultra Fast Preparative and Purification Liquid Chromatograph, all the work from preparation to enrichment, purification, and recovery is performed on-line. This significantly reduces the labor involved in the preparative purification process, and avoids procedural mistakes. Using a proprietary trap enrichment purification technology for the fractionated target components, trace quantities of components contained in mixtures can be recovered at high concentrations and high purity levels. Additionally, since highly volatile organic solvents are used for the recovery of target components, the time needed for evaporation to dryness can be significantly reduced. When recovering ionic target components, counter ions are flushed out by optimization of the solution flowing through, so the target component is recovered as a high purity free base. With these procedures, and the dedicated Purification Solution™ software, the entire process from the configuration of preparative conditions to the recovery of liquid containing the target compounds can be configured easily from simple and user friendly software.

 

Automation of the Preparative Purification Process

When target compounds are to be powderized, this process can be hindered by a number of factors including the presence of acids, salts, and refractory solvents. With the UFPLC Ultra Fast Preparative and Purification Liquid Chromatograph, these hindrances to powderization can be removed by flushing them out using a trap column, allowing a high purity powder of the target component to easily be obtained by anyone.

Even Trace Components Are Recovered at High Concentrations

Normally, when preparing trace quantity target components, preparative work involves injecting the sample multiple times to obtain sufficient amounts of the target. As a result, the volume of fractionation liquid ultimately obtained increases in proportion to the number of injections, increasing solvent use and dry down time. With the UFPLC Ultra Fast Preparative and Purification Liquid Chromatograph, even with multiple injections, the fraction of the target component is injected into the same trap column for enrichment. The final volume of fractionated liquid from the trap column is minimized, exchanging the weaker loading solvent for a suitable organic solvent. The target component can then be recovered at high purity levels and at high concentrations.

 

Purification Solution Simplifies Settings Related to Preparative Purification

The special Purification Solution software is equipped with peak tracking functions that enable the target peaks and fractionate and be checked at a glance.

 

Applications

Rmoval of Salts in the Mobile Phase Solvent
 

Removal of Ammonium Chloroacetate Salts from Ibuprofen

Ibuprofen, the target component, is cleaned by retention in a trap column. As a result, the ammonium chloroacetate salts contained in the mobile phase solvent were removed. This can prevent the retention of salts contained in the mobile phase solvent during powderization, so that only the target component is recovered.

Heightening the Efficiency of Enrichment Purification for Trace Components

High Speed Powderization of Cyclosporine A

The target compound fraction is repeatedly injected into the trap Ibuprofen column, enriched by trapping, and eluted by an organic solvent. This enables recovery with a smaller volume of liquid, so subsequent powderization can be performed in a shorter time. In this way, the same volume of powdered sample can be purified in a shorter time versus elution in reverse phase conditions.

 

 

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