Solutions for Oligonucleotide Analysis
During the last years, attention to biomolecules has been changed, the rapid development of a vaccines against Covid-19 based on a short mRNA sequence, has spurred the development of novel vaccines; The announcement of the potential impact of this concept on cancer therapy is leading to increased funding for these projects. Oligonucleotides, short sequences of nucleotides, are pivotal in various biotechnological and medical applications, including genetic testing, research, and therapeutic interventions. The analysis of oligonucleotides is crucial for ensuring their quality, efficacy, and safety in these applications.
Sequence Confirmation
Nucleic acid therapeutics have recently attracted particular attention as a new pharmaceutical modality. Unlike biopharmaceuticals, nucleic acid therapeutics can be produced by cost-effective chemical synthesis. The development and standardization of analytical methods for quality control are still in progress, with efforts being made in collaboration with industry and academia. In the development and evaluation of nucleic acid therapeutics, it is essential to understand their thermal stability, as this is a factor that controls their structure and functions. The confirmation whether or not synthesized nucleic acid therapeutics are arranged in the intended base sequence is also a critical quality characteristic to ensure the action of pharmaceuticals.
Puritiy Analysis
Nucleic acid drugs, such as antisense oligonucleotides, exert their efficacy by interacting with target genes inside and outside cells. Unlike conventional small molecule drugs, they are capable of targeting disease causes at the genetic level and are attracting attention as a next-generation drug. Nucleic acid drugs are mainly produced through chemical synthesis, but the synthesis process also produces many impurities such as shorter length components and protecting groups, so proper separation and purification of the target oligonucleotide is required.
More Insights with dedicated software
LabSolutions Insight Biologics is a software platform for analyzing the characteristics of oligonucleotides using single quadrupole (SQ) mass spectrometers, quadrupole time-of-flight (QTOF) mass spectrometers, or MALDI ionization mass spectrometers. Analyzing the characteristics of oligonucleotides involves the identification of impurities and sequence confirmation with modification positions estimated. Performing these analyses using a complicated mass spectrum requires a great deal of time. Additionally, using multiple software programs for the measurements and data analysis complicates the operations, and puts considerable strain on the operator. LabSolutions Insight Biologics was designed to resolve these issues.
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