Measuring Modes

Equipped with a variety of measurement modes and numerous functions as standard in a stand-alone instrument, the UV-1800 enables you to perform a range of applications, including photometrics and protein quantitation.

Photometric Mode

Measures the absorbance or transmittance at a single wavelength or at multiple (up to eight) wavelengths. In multiple-wavelength measurement, calculations can be performed on the data obtained for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths.

Spectrum Mode

Obtains sample spectra using wavelength scanning. Changes in the sample can be tracked using repeated scans. Data processing operations, such as the enlargement or reduction of the spectra obtained, peak detection, and area calculations, are also possible.

Spectrum Mode

Quantitation Mode

Generates a calibration curve from standard samples, and uses it to calculate the concentrations of unknown samples. Various combinations of wavelengths (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order regression calibration curves) are possible.

Quantitation Mode

Kinetics Mode

Measures the change in absorbance as a function of time, and thereby obtains enzymatic activity values. The kinetics measurement method or the rate measurement method can be selected. Using this mode in combination with an MMC-1600/1600C multi-cell holder (8/16 series multi-cell) or a CPS-240A cell positioner (6 cells) enables the consecutive analysis of multiple samples.

Kinetics Mode

Time-Scan Mode

Measures the change in absorbance, transmittance, or energy as a function of time. Using this mode in combination with an MMC-1600/1600C multi-cell holder (8/16 cells) or a CPS-240A cell positioner (6 cells) enables the simultaneous analysis of multiple samples.

Multi-Component Quantitation Mode

Separately quantitates up to eight components mixed in a single sample. Pure components or mixtures of components can be used as standard samples.

Biomethod Mode (provided as standard)

Capable of analyzing DNA and proteins using the following quantitation methods.

  • Nucleic Acid Quantitation- Quantitates DNA or protein using the absorbance at 260/230 nm or 260/280 nm.
  • Protein Quantitation
  • Lowry method
  • BCA method (using bicinchoninic acid)
  • CBB method (using Coomassie Brilliant Blue G-250)
  • Biuret method
  • UV absorption method (direct measurement at 280 nm)

Biomethod Mode

Security functions

Enabling the UV-1800 security functions makes it possible to restrict functions according to the user level.

Security functions

Instrument validation functions

The UV-1800 offers compatibility with 9 JIS items. Wavelength accuracy, wavelength repeatability, resolution, stray light, photometric accuracy, photometric repeatability,baseline flatness, baseline stability, noise level.

Instrument validation functions

Maintenance

The operating time of the deuterium (D2) lamp and the halogen (WI) lamp can be recorded and displayed. Consequently, the expected replacement period of the lamps can be ascertained when performing periodic inspections, minimizing downtime.

Maintenance

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