MSACL 2017

10.09.2017 - 14.09.2017
Salzburg, Austria
www.msacl.org

 

booth 1 & 2

 

Shimadzu is one of the worldwide leading manufacturers of analytical instrumentation. Its equipment and systems are used as essential tools in all areas of clinical research. Since more than 140 years, Shimadzu is at the service of science ensuring precise and reliable analyses. Among the leaders in GCMS as well as in LCMS, Shimadzu is offering a full range of LC-MS/MS systems to fit all needs depending on the application, and will introduce new solutions for screening and quantification in toxicology field. In addition, the unique and flexible fully automated sample preparation system seamlessly integrated with our LC-MS/MS range will be shown: CLAM-2000. Take the opportunity to visit our booth “1 & 2”.

 

 

Afternoon Workshops

 

Wednesday, 12. September 2017
14:00 - 14:30 h
Room Mozart 4-5 (Seats 125)

Disruptive Technologies and Creative Solutions in Clinical Mass Spectrometry

 

1I Practical application of the fully-automated sample preparation module, CLAM-2000, in clinical diagnostics
Dr. Thomas Stimpfl, Medical University of Vienna - Department of Laboratory Medicine

In clinical diagnostics random access to wide range of target analytes is needed. We will present our practical experiences with a novel, fully automated sample preparation module - directly connected to a LC-MS-system - in the routine setting of a laboratory in a university hospital.

2I Rethinking library identification in quantitative clinical toxicology – transitioning towards MRM Spectrum mode
Neil Loftus – SHIMADZU Corporation (UK), MS Business Unit

There are considerable opportunities in clinical mass spectrometry to bring about better innovations and better concepts to clinical laboratories. However, translating information rich mass spectrometry data into meaningful, actionable and safe test result within the context of a routine clinical laboratory remains a barrier for success for many clinical groups. To help the transition towards a more effective data review and higher confidence in reporting results we have been rethinking the capability of MRM in compound identification. In this presentation we will be headlining MRM Spectrum mode, an approach which can be set up in routine clinical labs helping to reduce false positive and false negative reporting without affecting quantitative data quality.

 

Register Now

 

 

Oral Presentation

 

Wednesday, 12. September, 14:45 h
Session: Endocrine
Mozart 4-5 - Track 2

 

Single Assay Measurement of the Aldosterone-Renin-Ratio by Online SPE-UHPLC-MS/MS
Presenter: Nicola GRAY - LCMS Application Specialist - SHIMADZU UK

 

 

Poster Presentations

 

Wednesday, 12. September, 9:45 h
Small Molecules

Poster # B1: The CLAM-2000 Challenge: Checking the Accuracy and Reproducibility of Automatic Sample Preparation of Steroid and Glucocorticoids for Plasma and Urine Samples
Jana Rykl, Shimadzu Schweiz AG

The accuracy and reproducibility of automatic sample preparation of steroid hormones and glucocorticoids for plasma and urine samples was compared with the results of manual sample preparation. Manual samples preparation of clinical samples normally include a protein precipitation step followed by centrifugation and/or extraction on SPE cartridges. Here we present an automatic sample preparation procedure using the CLAM-2000 robot coupled to an LCMS-8045 triple quadrupole mass spectrometer.

 

Wednesday, 12. September, 9:45 h
Small Molecules

Poster # D04: Evaluation of Blood Lysis Procedures prior to Automated Sample Preparation for Immunosuppressant Assay by LC-MS/MS
Ryu Konoshita, Shimadzu Europa GmbH

CLAM-2000 (Shimadzu Corp., Japan) fully automates blood or other samples pre-treatment prior to LC-MS analysis. For measurement of immunosuppressant (ISP) in whole blood samples, most target is bound to cytoplasmic proteins in erythrocytes and it is mandatory to lyse them prior to the pre-treatment. In this study, several protocols of hemolysis were evaluated based on lysis efficiency and ISP recovery. Freeze/thaw with -80‹C was efficient for both 0.2mL and 1mL. -20‹C was also effective but the reproducibility was very low. The efficiency and recovery rate by ammonium chloride was also good but sample dilution must be taken into account.

 

Wednesday, 12. September, 18:30 h
Small Molecules

Poster # E05: Fully Automated Platform for TDM Analysis in Serum Samples
Claudio Ghilardi, Shimadzu Italia

Therapeutic drug monitoring of several classes of drugs needs to be accomplished by extremely accurate techniques. Liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) shows higher sensitivity and superior specificity compared to immunoassay-based approaches. LC-MS/MS approaches lack in standardization since they require sample preparation procedures that usually involves complex offline extraction methods. These procedures are time-consuming and are more vulnerable to variability due to errors in manual preparation. To increase the data quality, safety, and throughput of LC-MS/MS quantitation of different drugs (Antiepileptics, Benzodiazepines, Neuroleptics, Tricyclic Antidepressant), a fully automated LCMS/MS platform has been introduced.

 

Wednesday, 12. September, 18:30 h
Small Molecules

Poster # B22: A novel fast and simple quantification method for bile acids in human serum by LC-MS/MS.
Aurore Jaffuel, Shimadzu France

Bile Acids (BAs) are steroid acids of the bile. They are fat substances solubilizers of the enterohepatic cycle and are key players of lipid and energy metabolism. Abnormal BAs profiles may be used as biomarkers of several liver diseases. Despite the clinical need of measuring BAs concentration in plasma/serum, reference separations were proved to be unsuitable for simple and rapid analysis. Here is proposed a new LC-MS/MS method for the simultaneous high sensitive quantification of 27 BAs. A quick sample preparation technique based on proteins precipitation was applied. Good repeatability was obtained (RSD<5%). Linearity was tested for 16 BAs and confirmed for all in the range 1 to 100 ng/ml. The r2 coefficients were above 0.99, with S/N>10 for LLOQ levels.

 

Wednesday, 12. September, 18:30 h
Small Molecules

Poster # B26: Quantitative toxicology screening for over 1000 compounds in whole blood samples; is there a better way of reporting results?
Stephane Moreau, Shimadzu Europa GmbH

Clinical pathology laboratories use highly specific, sensitive methods and technologies working across a multi-disciplinary teams delivering actionable data. However, if a false positive result is reported the downstream impact to unnecessary testing and treatment can be considerable. In this paper we present a library of product ion spectra for 1222 compounds that has been developed for clinical and forensic toxicology screening to help reduce false positive and false negative reporting. The library enables multi-targeted methods to be developed for routine screening, library identification and quantitation.

 

Wednesday, 12. September, 18:30 h
Endocrinology

Poster # F03: Quantitation of plasma metanephrine and normetanephrine by derivatization using LC-MS/MS analyzer integrated with fully-automated sample preparation device.
Atsuhiko Toyama, Marketing Innovation Centre, Shimadzu Corporation

Although LC-MS/MS analysis manifests high repeatability in measurement, overall reproducibility of an assay is compromised by errors associated with manual sample pretreatment. This also hinders standardization of assay across multiple laboratories. In this investigation, we evaluated whether automated sample preparation device can be employed to carry out complex sample pretreatment procedures, such as required for chemical derivatization. As model experiment, plasma metanephrine and normetanephrine was derivatized by reductive amination to improve reversed phase column retention.

 

Thursday, 13.September, 10:00h
Small Molecules

Poster # A4: Fully Automatized LC-MS/MS Analysis of Neuroleptics Using a Novel Sample Preparation System
Sigrid Baumgarten, Shimadzu Europa

The precise quantitation of Neuroleptics for therapeutic purpose is necessary. To avoid the sample preparation which is often tedious, involves risk of errors and increases the risk of contamination of people dealing with sample preparation The CLAM-2000 (Clinical Laboratory Automated sample preparation Module) automates the pretreatment of blood or other biological samples before LCMS analysis. By simply placing blood collection tubes in the system, the CLAM-2000 performs all processes through to LCMS analysis automatically. A complete neuroleptics analysis of Neuroleptic kit from Chromsystems was demonstrated.

 

Thursday, 13.September, 10:00h
Small Molecules

Poster # B16: Rethinking library identification in quantitative clinical toxicology – transitioning towards MRM Spectrum mode
Neil Loftus, Shimadzu Corporation

There are considerable opportunities in clinical mass spectrometry to bring about better innovations and better concepts to clinical laboratories. To help transition towards a more effective data review and higher confidence in reporting results we have been rethinking the capability of MRM in compound identification. In this poster we will show a different approach in identifying compounds by MRM Spectrum mode, an approach which can be set up in routine clinical labs helping to reduce false positive and false negative reporting without affecting quantitative data quality.

 

Thursday, 13.September, 10:00h
Small Molecules

Poster # A4: Fully Automatized LC-MS/MS Analysis of Neuroleptics Using a Novel Sample Preparation System
Sigrid Baumgarten, Shimadzu Europa

The precise quantitation of Neuroleptics for therapeutic purpose is necessary. To avoid the sample preparation which is often tedious, involves risk of errors and increases the risk of contamination of people dealing with sample preparation The CLAM-2000 (Clinical Laboratory Automated sample preparation Module) automates the pretreatment of blood or other biological samples before LCMS analysis. By simply placing blood collection tubes in the system, the CLAM-2000 performs all processes through to LCMS analysis automatically. A complete neuroleptics analysis of Neuroleptic kit from Chromsystems was demonstrated.

 

 

Product launch

 

Toxicology Database and Insight Screening software

See details in the presentation in the workshop
 

 

Product Lineup

 

  • LCMS-8060 Triple Quadrupole Mass Spectrometer

The LCMS-8060 is designed to push the limits of LC-MS/MS quantitation for applications requiring the highest sensitivity and robustness while delivering a meaningful solution for routine LC-MS/MS analyses.

  • CLAM-2000 Fully automated sample preparation

The CLAM-2000 (RUO) is a new and unique fully automated sample preparation module connected online to LC-MS/MS. It automatizes the pretreatment of blood or other biological samples prior to LC-MS/MS analysis.