Ultra-High Sensitivity/Ultra-High Speed(2)

Higher Sensitivity for Q1 Full Scan Spectra

The LCMS-8040 maintains the same high-speed scanning (UFscanning) and polarity switching technology (UFswitching) utilized in the LCMS-8030.

The LCMS-8040 not only maintains Shimadzu's proprietary high-speed technologies (UF Technologies, Patent pending), which minimize sensitivity losses even at faster scan speeds, it also features improved ion optics, which provide higher sensitivity for MRMs and full scans. A comparison of Q1 full scan spectra for pesticide samples (Methomyl, Carbaryl (NAC), Phoxim, Benfuracarb,and Abamectin B1a) is shown below. The upper spectra were acquired using the LCMS-8040 and the lower spectra were acquired using the LCMS-8030. As shown, the LCMS-8040 offers significant sensitivity improvements for precursor ions or full scan data.

Higher Sensitivity for MS/MS Acquisition Modes

The increased scan sensitivity of the LCMS-8040 also applies to scan modes specific to triple quadrupole mass spectrometers, such as product ion scanning, precursor ion scanning, and

neutral loss scanning. Historically, mass spectrometers have introduced mass deviation in linked scans, such as precursor ion scans or neutral loss scans, when measured at maximum scan speeds.

However, Shimadzu's proprietary UFscanning technology allows the user to perform precursor ion scans or neutral loss scans at high speeds without sacrificing the quality of spectra. In addition, the LCMS-8040 offers higher sensitivity levels. Precursor scan results for eight kinds of phthalate esters are shown to the left. Scans were performed at two speeds, 2727 u/sec and 6000 u/sec. No mass shift is observed at either scan speed, and a significant sensitivity improvement is observed for the LCMS-8040.

Exceptional Durability

Blood plasma samples were spiked with verapamil and warfarin, and then protein precipitation was carried out according to the pretreatment flow indicated below. The area values from 450 consecutive LCMS-8040 analyses were then plotted. Simultaneous analysis of verapamil by ESI+ and warfarin by ESI− was performed. Chromatograms for the 1st, 250th, and 450th measurements are shown below. This resulted in 1 pg on-column area repeatability of 4.18% for verapamil and 6.61% for warfarin.

Ultra Fast Separation Combined with Lower Femtogram Detection

Using the same UHPLC method and samples as described above, 100 fg of verapamil and warfarin was injected into the LCMS-8040. For 100 fg on-column, a S/N ratio of 146 (rms) was obtained for verapamil and 30 (rms) for warfarin.

The resulting lower limits of detection for S/N = 3 (rms) were 2.05 fg for

verapamil and 9.88 fg for warfarin

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