CLAM-2000-LC-MS/MS Application Examples
CLAM-2000-LC-MS/MS Application Examples
Steroids
This analysis flow is an example of a simultaneous multicomponent analysis of steroids in serum using a fully automated preparative LCMS analysis system consisting of a Nexera X2 Ultra High Performance Liquid Chromatograph, LCMS-8060 Triple Quadrupole Mass Spectrometer, and CLAM-2000 fully automated sample preparation module for LCMS.
Pretreatment Conditions and Analysis Flow
Quantitative results of drug spiked into serum
| Compounds | Polarity | Range (ng/mL) |
r2 | QC samples concentration (ng/mL) | Accuracy (%) | %RSD (n=3) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LLOQ | Middle | ULOQ | LLOQ | Middle | ULOQ | LLOQ | Middle | ULOQ | ||||
| Aldosterone | + | 0.03 - 1.14 | 0.997 | 0.03 | 0.46 | 1.14 | 96.5 | 102.0 | 102.8 | 6.3 | 7.9 | 3.6 |
| Cortisol | + | 1.51 - 320 |
0.999 | 15.1 | 20.8 | 320 | 104.4 | 100.4 | 98.6 | 1.6 | 0.5 | 0.7 |
| DHEAS | + | 12.9 - 2750 |
0.999 | 12.9 | 179 | 2750 | 104.3 | 101.3 | 99.6 | 3.9 | 3.6 | 1.9 |
| Corticosterone | + | 0.29 - 62 |
0.998 | 0.29 | 4.03 | 62 | 101.8 | 101.0 | 98.4 | 4.6 | 0.8 | 1.0 |
| 11-Deoxycortisol | + | 0.08 - 18 |
0.997 | 0.08 | 1.17 | 18 | 103.0 | 102.7 | 96.1 | 5.5 | 0.9 | 0.2 |
| Androstenedione | + | 0.08 - 18 |
0.999 | 0.08 | 1.17 | 18 | 116.5 | 99.1 | 100.1 | 4.6 | 1.3 | 1.1 |
| Testosterone | + | 0.03 - 7.2 |
0.999 | 0.03 | 0.47 | 7.2 | 93.8 | 101.4 | 100.1 | 3.7 | 1.0 | 1.0 |
| 17-OHP | + | 0.12 - 26 |
0.998 | 0.12 | 1.69 | 26 | 103.6 | 103.3 | 99.6 | 6.2 | 1.6 | 1.8 |
| DHEA | + | 0.31 - 65 |
0.999 | 0.31 | 4.22 | 65 | 93.1 | 98.1 | 99.0 | 6.5 | 3.9 | 6.5 |
| Progesterone | + | 0.12 - 26.5 |
0.999 | 0.12 | 1.72 | 26.5 | 103.3 | 101.0 | 99.2 | 3.9 | 1.1 | 1.5 |
| Aldosterone (neg) | - | 0.03 - 1.14 |
0.996 | 0.03 | 0.46 | 1.14 | 115.0 | 97.3 | 97.4 | 6.6 | 7.5 | 8.0 |
| DHEAS (neg) | - | 12.9 - 444 |
0.997 | 12.9 | 20.8 | 444 | 101.7 | 103.0 | 99.7 | 2.1 | 1.2 | 7.1 |
For Research Use Only. Not for use in diagnostic procedures. Not approved as medical device by U.S. FDA. This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.