CLAM-2000-LC-MS/MS Application Examples

CLAM-2000-LC-MS/MS Application Examples

Steroids

This analysis flow is an example of a simultaneous multicomponent analysis of steroids in serum using a fully automated preparative LCMS analysis system consisting of a Nexera X2 Ultra High Performance Liquid Chromatograph, LCMS-8060 Triple Quadrupole Mass Spectrometer, and CLAM-2000 fully automated sample preparation module for LCMS.

Pretreatment Conditions and Analysis Flow

Quantitative results of drug spiked into serum

Compounds Polarity Range
(ng/mL)
r2 QC samples concentration (ng/mL) Accuracy (%) %RSD (n=3)
LLOQ Middle ULOQ LLOQ Middle ULOQ LLOQ Middle ULOQ
Aldosterone + 0.03 - 1.14 0.997 0.03 0.46 1.14 96.5 102.0 102.8 6.3 7.9 3.6
Cortisol + 1.51 -
320
0.999 15.1 20.8 320 104.4 100.4 98.6 1.6 0.5 0.7
DHEAS + 12.9 -
2750
0.999 12.9 179 2750 104.3 101.3 99.6 3.9 3.6 1.9
Corticosterone + 0.29 -
62
0.998 0.29 4.03 62 101.8 101.0 98.4 4.6 0.8 1.0
11-Deoxycortisol + 0.08 -
18
0.997 0.08 1.17 18 103.0 102.7 96.1 5.5 0.9 0.2
Androstenedione + 0.08 -
18
0.999 0.08 1.17 18 116.5 99.1 100.1 4.6 1.3 1.1
Testosterone + 0.03 -
7.2
0.999 0.03 0.47 7.2 93.8 101.4 100.1 3.7 1.0 1.0
17-OHP + 0.12 -
26
0.998 0.12 1.69 26 103.6 103.3 99.6 6.2 1.6 1.8
DHEA + 0.31 -
65
0.999 0.31 4.22 65 93.1 98.1 99.0 6.5 3.9 6.5
Progesterone + 0.12 -
26.5
0.999 0.12 1.72 26.5 103.3 101.0 99.2 3.9 1.1 1.5
Aldosterone (neg) - 0.03 -
1.14
0.996 0.03 0.46 1.14 115.0 97.3 97.4 6.6 7.5 8.0
DHEAS (neg) - 12.9 -
444
0.997 12.9 20.8 444 101.7 103.0 99.7 2.1 1.2 7.1

For Research Use Only. Not for use in diagnostic procedures. Not approved as medical device by U.S. FDA. This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.

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