High Performance for a Variety of Applications

Veterinary Drugs

 

 

Nitrofuran metabolites in fish

 

 

Nitrofurans are a class of synthetic antibiotics. The use of nitrofurans for edible animal and fishery products is banned in many countries. The chromatogram below shows the recovery of nitrofuran metabolites from fish using the LCMS-8045. Excellent linearity and accurate quantitative results ranging from 0.05 to 20 ng/mL were obtained. The LCMS-8045 is shown to be an excellent platform for routine high-sensitivity analysis in challenging matrices.

 


MRM chromatogram of nitrofuran metabolites spiked in fish (0.5ng/mL)
 
Compound Calibration range
(ng/mL)

Accuracy

(%)

LOD
(ng/mL)
AMOZ 0.05 - 20 94.5 - 103.8 0.00024
SEM 0.05 - 20 93.2 - 111.6 0.015
AHD 0.05 - 20 95.5 - 102.1 0.001
AOZ 0.05 - 20 94.0 - 108.4 0.00096

*Limit of detection (LOD) was calculated at signal-to-noise ratio (S/N) = 3.

 

Residual Pesticides

 

 

Simultaneous analysis of residual pesticides in herbal supplements

 

 

Regulatory agencies worldwide provide oversight for an extensive and ever-increasing list of pesticides in order to promote food safety and protect public health. The MRM chromatogram below shows the simultaneous analysis of 153 pesticides spiked into Codonopsis pilosula at 0.01 mg/kg. Plant matrices can be very challenging due to background interferences, but the LCMS-8045 provides enough sensitivity to analyze most pesticides at the trace level of 10 parts per billion. Many pesticides methods also benefit from polarity switching, which the LCMS-8045 can accomplish in just 5 msec.

 


153 regulated pesticides spiked into Codonopsis pilosula at 10 parts per billion

 

 

 

Use Synchronized Survey Scan (SSS) to obtain both qualitative and quantitative information from a single injection. Full scan MS2 spectra will be obtained when an MRM peak intensity threshold is exceeded.

 

Water Quality Analysis

 

 

High-sensitivity analysis of 21 pharmaceuticals and personal care products in less than eight minutes

 

 

Pharmaceutical and personal care products (PPCPs) encompass a family of compounds used by individuals for health and cosmetic purposes. As populations increase, and the use of these products increases, PPCPs will become a significant threat to our water supply. The LCMS-8045 is capable of trace-level quantitation of many PPCPs without extensive sample preparation.

 


MRM chromatograms of 21 PPCPs (1.0 ng/mL)

No. Compound LOD
(ng/mL)
LOQ
(ng/mL)
No. Compound LOD
(ng/mL)
LOQ
(ng/mL)
1 Lincomycin 0.01 0.04 12 Ifenprodil 0.0075 0.022
2 Trimethoprim 0.01 0.04 13 Erythromycin 0.015 0.045
3 Pirenzepine 0.01 0.03 14 Azithromycin 0.01 0.03
4 Ofloxacin 0.01 0.03 15 Clarithromycin 0.0034 0.01
5 Ciprofloxacin 0.002 0.006 16 Roxithromycin 0.0071 0.022
6 Sulphapyridine 0.002 0.006 17 Carbamazepine 0.01 0.03
7 Carbazochrome 0.015 0.045 18 DEET 0.0062 0.018
8 Metoprolol 0.013 0.04 19 Crotamiton 0.005 0.015
9 Dicorantil 0.005 0.015 20 Bezafibrate 0.0025 0.075
10 Sulfamethoxazole 0.003 0.01 21 Triclocarban 0.01 0.03
11 Antipyrine 0.01 0.03        

*Limit of detection (LOD) was calculated at signal-to-noise ratio (S/N) = 3.

Limit of quantitation (LOQ) was calculated at S/N = 10.

Bioanalysis

 

 

High-sensitivity quantitation of Amlodipine in plasma

 

 

Amlodipine is widely utilized for the treatment of high blood pressure and angina. A high-sensitivity method for the quantitation of amlodipine in human plasma using LCMS-8045 has been developed. Appropriate linearity ranging from 0.03 to 300 ng/mL with excellent accuracy was achieved. Both interday and intraday variabilities were assessed for quality control samples. Percent RSD (Relative Standard Deviation) results were less than 5%.

 

Quantitative Results of Amlodipine
 

Lv Actual conc.
(ng/mL)
Calculated Conc.
(ng/mL)

Accuracy

(%)

1 0.003 0.003 104.5
2 0.01 0.01 97.2
3 0.03 0.031 103
4 0.1 0.107 107.1
5 0.3 0.268 89.4
6 1 1.062 106.2
7 3 2.958 98.6
8 10 10.293 102.9
9 30 27.37 91.2
10 100 98.722 98.7
11 300 303.619 101.2

 

 

Interday and intraday variations of Amlodipine spiked in human plasma
 

QC sample
Concentration

(ng/mL)

%RSD

accuracy

(%)

Interday
Variation

(n=6)

Low 0.1 3.78 97.8-109.1
Middle 5 3.16 90.0-95.4
High 240 0.85 95.9-97.6
Intraday
Variation

(n=18)

Low 0.1 4.5 87.5-110.3
Middle 5 4.92 88.1-102.9
High 240 4.25 88.3-104.0

 

 

For Research Use Only. Not for use in diagnostic procedures.

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