Size Exclusion Columns

Shim-pack GPC Series

Shim-pack GPC series columns are used for the determination of tetrahydrofuran (800 Series), chloroform (800C Series), and dimethylformamide (800D series). The technique of GPC does not utilize such chemical reactions as partition, adsorption, and ion exchange, but a physical reaction consisting of a separation based on molecular size of the sample components. Therefore, this method is suitable for the measurement of molecular weight distribution of high polymers and oligomers. Shim-pack GPC series are packed with polystyrene polymers with respective degrees of cross-linking in order to meet exact analysis requirements, ranging from analysis of high polymers to that of oligomers. GPC-80M (80MC, 80MD) are mixed gel columns.

Analysis Examples

Calibration Curves

■Conditions

Mobile Phase: Tetrahydrofuran
Flow Rate: 1.0 mL/min
Col. Temp.: Ambient
Detection: UV 254 nm

Analysis of Polystyrene Standard

■Conditions

Column: Shim-pack GPC-80M (2 columns in series)
(P/N: 228-20810-91)
Mobile Phase: Tetrahydrofuran
Flow Rate: 1.0 mL/min
Col. Temp.: Ambient
Detection: UV 254 nm

Determination in Tetrahydrofuran

Column Exclusion Limit
(polystyrene)
Dimensions
(Length x I.D., mm)
P/N
Shim-pack GPC-801 1.5 x 103 300 x 8.0 228-20803-91
Shim-pack GPC-802 5 x 103 300 x 8.0 228-20804-91
Shim-pack GPC-8025 2 x 104 300 x 8.0 228-20805-91
Shim-pack GPC-803 7 x 104 300 x 8.0 228-20806-91
Shim-pack GPC-804 4 x 105 300 x 8.0 228-20807-91
Shim-pack GPC-805 4 x 106 300 x 8.0 228-20808-91
Shim-pack GPC-806 4 x 107 300 x 8.0 228-20809-91
Shim-pack GPC-80M 4 x 107,Mixed gel 300 x 8.0 228-20810-91
Shim-pack GPC-800P Guard Column 10 x 4.6 228-20812-91


 

Determination in Dimethylformamide

Column Exclusion Limit
(polystyrene)
Dimensions
(Length x I.D., mm)
P/N
Shim-pack GPC-801D 1.5 x 103 300 x 8.0 228-20803-93
Shim-pack GPC-802D 5 x 103 300 x 8.0 228-20804-93
Shim-pack GPC-8025D 2 x 104 300 x 8.0 228-20805-93
Shim-pack GPC-803D 7 x 104 300 x 8.0 228-20806-93
Shim-pack GPC-804D 4 x 105 300 x 8.0 228-20807-93
Shim-pack GPC-805D 4 x 106 300 x 8.0 228-20808-93
Shim-pack GPC-806D 4 x 107 300 x 8.0 228-20809-93
Shim-pack GPC-80MD 4 x 107,Mixed gel 300 x 8.0 228-20810-93
Shim-pack GPC-800DP Guard Column 10 x 4.6 228-20812-93

Shim-pack Diol Series

Shim-pack Diol series is a kind of gel filtration chromatography (GFC) column. GFC is used to separate water-soluble high polymers such as polysaccharides, proteins, and nucleic acids according to their molecular sizes by using hydrophilic packing materials and aqueous mobile phase. Shim-pack Diol series is packed with porous spherical silica gel chemically bonded with a hydroxyl group. Due to the hydrophilic hydroxyl group, Shim-pack Diol series can be used in high-speed GFC and provide sharp peaks during the analysis of protein and biochemicals (such as enzymes).

Analysis Examples

Calibration Curves

■Peaks

  1. Blue dextran 2000
  2. Thyroglobulin
  3. y-Globulin
  4. Transferrin
  5. Human serum albumin
  6. Ovalbumin
  7. β-Lactoglobulin
  8. Chymotrypsin
  9. Myoglobin
  10. Lysozyme
  11. Ribonuclease A
  12. Cytochrome C
  13. Gly-Tyr

■Conditions

Column: Shim-pack Diol Series (500 mmL × 7.9 mmI.D., 5 μm)
Mobile Phase: A) 10mM phosphate buffer solution (pH 7)
B) 0.2M sodium sulfate
Flow Rate: 1.0 mL/min
Col. Temp.: Ambient
Detection: UV 280 nm

Analysis of Protein Standard

■Peaks

  1. Glutamate dehydrogenase
  2. Lactate dehydrogenase
  3. Enolase
  4. Adenylate kinase
  5. Cytochrome C

■Conditions

Column: Shim-pack Diol-300 (500 mmL × 7.9 mmI.D., 5 μm)
(P/N: 228-14776-92)
Mobile Phase: A) 10mM phosphate buffer solution (pH 7)
B) 0.1M sodium chloride
Flow Rate: 1.0 mL/min
Col. Temp.: Ambient
Detection: UV 280 nm

Product Information

Column Stationary
phase
Particle Size
(µm)
Dimensions
(I.D. x Length, mm)
P/N
Shim-pack Diol-150 Diol group 5 7.9 x 250 228-14775-91
7.9 x 500 228-14775-92
Shim-pack Diol-300 Diol group 5 7.9 x 250 228-14776-91
7.9 x 500 228-14776-92
Pre-column Diol * Diol group 10 4.0 x 50 228-16367-91

* Installed between the liquid pump and the sample injector to protect Shim-pack Diol column.

For Research Use Only. Not for use in diagnostic procedures.

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